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Image Search Results
Journal: eLife
Article Title: Calibration of cell-intrinsic interleukin-2 response thresholds guides design of a regulatory T cell biased agonist
doi: 10.7554/eLife.65777
Figure Lengend Snippet:
Article Snippet: For quantitative polymerase chain reaction (qPCR), Tregs and CD8+ T cells were stimulated for 4 hr with 200 nM cytokine prior to RNA isolation using RNeasy kit (
Techniques: Expressing, Recombinant, Modification, Sequencing, Cell Isolation, Selection, Reverse Transcription, SYBR Green Assay, Flow Cytometry, Staining, Activation Assay, Software